Chikungunya virus (CHIKV) infection: Analytical performance of real-time NASBA assay

Challenges in Surveillance for Chikungunya Virus (CHIKV) Infection

Introduction CHIKV is transmitted by mosquitoes and often occurs in large outbreaks with high attack rates. Common symptoms (which can be severe and disabling) include fever, joint pain/swelling, headache, muscle pain and rash. In December 2013, the World Health Organization reported local CHIKV transmission in the Caribbean. In July 2014, the first locally-acquired case in the continental U.S....

Why Can We See a Local Spread of Chikungunya Virus (CHIKV) Infection?

Real-Time NASBA

NASBA is an isothermal nucleic acid amplifi cation method that is particularly suited to detection and quantifi cation of genomic, ribosomal or messenger RNA. The product of NASBA is single-stranded RNA of opposite sense to the original target. The fi rst developed NASBA methods relied on liquid or gel-based probe-hybridisation for post-amplifi cation detection of products. More recently, real-...

Nonhuman Primate Models of Chikungunya Virus Infection and Disease (CHIKV NHP Model)

Chikungunya virus (CHIKV) is a positive-sense RNA virus transmitted by Aedes mosquitoes. CHIKV is a reemerging Alphavirus that causes acute febrile illness and severe and debilitating polyarthralgia of the peripheral joints. Huge epidemics and the rapid spread of CHIKV seen in India and the Indian Ocean region established CHIKV as a global health concern. This concern was further solidified by ...

Analytical and Clinical Performance of a Real-time Screening PCR Assay Identifying Congenital CMV Infection

1164. Influenza Clinical Diagnostic Testing and Antiviral Treatment among Children Hospitalized with Acute Respiratory Illness During the 2015–16 Influenza Season Angela P. Campbell, MD, MPH, FPIDS, FIDSA; Craig McGowan, MS; Brian Rha, MD, MSPH; Julie A. Boom, MD; Janet Englund, MD, FIDSA; Natasha B. Halasa, MD, MPH, FPIDS; Rangaraj Selvarangan, PhD; Mary A. Staat, MD, MPH; Geoffrey A. Weinberg...